Tdtomato reporter mice TdTomato is expressed in the skin upon crossing of the reporter mice with a K5-Cre-ERT2 line. Brain, epidermis, dermis, liver, lung, kidney, pancreas, heart, salivary gland Nov 20, 2022 · The Neh2-Cre transgenic mouse was crossed with the ROSA26-loxP-stop-loxP-tdTomato reporter mouse (ROSA-LSL-tdTomato mouse). In order to provide an in vivo tool to improve our understanding of lymphatic vessel function in physiological and pathological conditions, we generated and characterized a tdTomato reporter mouse and crossed it with a mouse line expressing Cre recombinase under the control ProxTom lymphatic vessel reporter mice reveal Prox1 expression in the adrenal medulla, megakaryocytes, and platelets . 007909 B6. The compound mutant reporter mice exhibited accumulation of tdTomato-positive cells in various organs after repeated administration of CDDO-Im, one of the NRF2-inducing electrophiles. Ai14 mice express robust tdTomato fluorescence following Cre-mediated recombination. These mice express robust tdTomato fluorescence following Cre-mediated recombination. SCGE ID: 13000000038: Name: Ai14 mouse: Official Name: B6;129S6-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze /J: Species: Mouse: Type: Animal: Description: Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The applications of reporter mouse models are various. (B) Flow cytometric analysis of tdTomato expression in CD45-tissue cells in Ms4a3 Cre-Rosa TdT mice. The mice were also successfully used May 19, 2023 · Herein, we report a MyoD knock-in (MyoD-KI) reporter mouse expressing tdTomato at the endogenous MyoD locus. In Cre-expressing cells, the Stop signal was irreversibly removed and tdTomato expression was induced. We further compared LysM-cre tdTomato recombination with that of Cdh5-cre driver, which is expressed in both endothelial and hematopoietic cells. By placing a reporter cassette (GSG-3xFlag-P2A-tdTomato) between the coding sequence of exon 2 and 3'UTR of the Tmem119 gene using CRISPR/Cas9 technology, we generated a Tmem119-tdTomato knock-in mouse strain. Prox1: Prox1-EGFP BAC: EGFP: Jung: 2017 Nov 17, 2023 · Here, we have created a new mouse strain in which the Cre recombinase gene was inserted into the RBPMS locus using CRISPR-Cas9 technology; the mice were crossed with Ai9 tdTomato reporter mice, resulting in the creation of RBPMS CreERT2-tdTomato mice, within the tdTomato protein fluorescence signals reflecting the endogenous expression of RBPMS Sep 5, 2019 · Ms4a3 Cre mice were crossed with Rosa tdTomato reporter mice. The Ai14 allele is very similar in design to the Ai9 allele (Stock No. 007914 B6. The lymphatic vascular system plays an active role in immune cell trafficking, inflammation and cancer spread. 007909 ) - differing only in the absence (Ai14) or presence (Ai9) of an att site-flanked neo selection cassette at the 3' end of the targeted allele. Cg- Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze /J Ai14 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Apr 19, 2018 · Dong et al 73 targeted exogenous genes into the ROSA26 locus and generated a ratiometric tdTomato-GCaMP6f reporter mouse which can be applied in visualizing T-cell calcium dynamics. Apr 2, 2021 · Description: This mouse reporter line harbors a lox-P flanked STOP cassette preventing transcription of a CAG promoter driven red fluorescent protein (tdTomato) in the Rosa26 locus. Jul 12, 2018 · Modern genetic approaches are powerful in providing access to diverse cell types in the brain and facilitating the study of their function. Nov 20, 2022 · The Neh2-Cre transgenic mouse was crossed with the ROSA26-loxP-stop-loxP-tdTomato reporter mouse (ROSA-LSL-tdTomato mouse). Additionally, we showed that tdTomato fluorescence intensity defines MuSC Download scientific diagram | Ai9 Cre Reporter Mouse Strain and Study Plans for AAV Local and Systemic Delivery (A) Ai9 Cre reporter mouse strain with tdTomato expression induced by Cre . 这个STOP盒子使得tdTomato基因在没有Cre重组酶的情况下不会表达。 当Ai14小鼠与表达Cre重组酶的小鼠交配时，Cre重组酶在其表达的细胞中会切除STOP盒子，使得这些细胞开始表达tdTomato红色荧光蛋白。这样，研究人员就可以通过红色荧光来标记和追踪这些细胞。 Jan 2, 2022 · 图1. tdtomato的打靶策略[1] Mar 2, 2018 · We hypothesized that in vivo delivery of mRNA could be more easily visualized in a genetically modified mouse with a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven tdTomato protein in all cells, such as the Ai14 reporter mouse (Figure 1 A). aureus and guide RNAs specific for the stop signal flanking regions of the Ai9 allele were injected intravenously into male mice homozygous for the reporter allele. 所谓 reporter鼠，就是敲入了报告基因（reporter gene）的鼠。 常见的有 TdTomato鼠 、 mT/mG鼠 、 LacZ鼠 、彩虹鼠 等，他们的重要作用是对各种特异性表达的Cre工具鼠进行验证，简而言之就是“工具鼠的工具鼠”：前面我们关于 Cre-loxp系统 的介绍中大家已经知道了带有特异性表达的Cre工具鼠，然而如何去验证这种鼠的特异性表达呢？ 前人们巧妙地设计了 reporter鼠来指示Cre鼠的特异性。 B-tdTomato cKI大鼠. A Schematic representation of the breeding to K5-Cre-ERT2 and of 4-hydroxytamoxifen (4-OHT Abstract. Cg- Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Ai9 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Download scientific diagram | Ai14/Cre mRNA Mouse Model Description and Lipid Nanoparticle Characterization (A) Diagram of the loxP-flanked STOP cassette upstream of tdTomato with and without Cre Sep 19, 2019 · Mouse reporter strains to track temporal changes in cytokine expression in Th17 and Treg cells were generated by crossing a well-characterized transgenic mouse that carries a constitutively expressed floxed-stopped tdTomato reporter gene to mice expressing Cre recombinase under the control of either the il17a or Foxp3 promoters , respectively. 26 In this model, cells that are successfully transfected with mRNA encoding Cre recombinase (Cre) would excise the loxP Mar 9, 2020 · Methods: We examined LysM-cre recombination in retinal tissue using a LysM-cre/tdTomato reporter mouse together with immunolabeling for several retinal cell markers. Expression of tdTomato in MyoD-KI mice recapitulated the endogenous MyoD expression dynamics in vitro and during the early phase of regeneration in vivo. Prox1: Prox1-Cre-tdTomato: tdTomato: Bianchi: 2015: A Transgenic Prox1-Cre-tdTomato Reporter Mouse for Lymphatic Vessel Research . SD-ROSA26-LSL-tdTomato大鼠（产品编号：CR003） 是一种条件性激活红色荧光的Reporter模型。在该模型中，tdTomato基因表达元件被整合到ROSA26安全位点，并受CAG启动子的调控。正常情况下，其表达会被上游的LSL元件阻断，但可以通过Cre重组酶去除LSL元件以激活tdTomato的表达。 SD-ROSA26-LSL-tdTomato大鼠（产品编号：CR003）是一种条件性激活红色荧光的Reporter模型。在该模型中， tdTomato基因 表达元件被整合到ROSA26安全位点，并受CAG启动子的调控。正常情况下，其表达会被上游的LSL元件阻断，但可以通过Cre重组酶去除LSL元件以激活tdTomato的表达。 前人们巧妙地设计了reporter鼠来指示cre鼠的特异性。 b-tdtomato cki大鼠 tdtomato大鼠 在rosa位点敲入了一个cag启动子、tdtomato蛋白编码序列和wpre序列，并在cag启动子和tdtomato序列之间插入了lsl（loxp-3×stop-loxp）序列。如图1所示。 图1. Jan 1, 2020 · By placing a reporter cassette (GSG-3xFlag-P2A-tdTomato) between the coding sequence of exon 2 and 3′UTR of the Tmem119 gene using CRISPR/Cas9 technology, we generated a Tmem119-tdTomato knock-in mouse strain. TdTomato的打靶策略[1] 这种鼠在正常情况下由于3×stop的存在并不能表达红色荧光，当与Cre鼠或TAM激活的CreERT2鼠交配后，产生的后代由于Cre重组酶切割loxp位点之间的序列将3×stop切除，TdTomato得到表达，就可以在Cre或CreERT2表达的组织中发出红色荧光，指示基因的位置。 Rosa-CAG-LSL-tdTomato-WPRE条件等位基因的半合子小鼠是可育的。 一侧带有loxp的STOP元件阻止下游红色荧光蛋白变体(tdTomato)的转录。 由于这种CAG启动子驱动的表达元件构建是靶向插入Gt(ROSA)26Sor位点的，因此tdTomato的表达取决于表达Cre重组酶的组织。 Apr 7, 2015 · In order to provide an in vivo tool to improve our understanding of lymphatic vessel function in physiological and pathological conditions, we generated and characterized a tdTomato reporter mouse and crossed it with a mouse line expressing Cre recombinase under the control of the lymphatic specific promoter Prox1 in an inducible fashion. Here, we report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. Importantly, both Ai14 and Ai9 may Aug 21, 2021 · tdTomato鼠，在ROSA位点敲入了一个CAG启动子、tdTomato蛋白编码序列、WPRE序列，并在CAG启动子和tdTomato序列之间插入了LSL（LoxP-Stop-LoxP）序列（图）[1]。 tdTomato鼠 在正常情况下，由于Stop的存在，tdTomato并不能表达红色荧光。 Nov 20, 2022 · The Neh2-Cre transgenic mouse was crossed with the ROSA26-loxP-stop-loxP-tdTomato reporter mouse (ROSA-LSL-tdTomato mouse). Apr 7, 2015 · Since the Prox1-Cre-tdTomato mouse features two alleles transmitted independently (the Prox1-driven Cre recombinase and the inducible tdTomato reporter), this application will require the generation of triple-transgenic mice. Mar 16, 2015 · After we crossed Catchup mice with a reporter line expressing high levels of tdTomato from a Cre-activatable CAG promoter in the ROSA26 locus 20 (these mice were termed Catchup IVM-red, as they Oct 28, 2024 · CRISPR/Cas-mediated activation of tdTomato in adrenal glands of Ai9 mice: Adeno-associated viruses (serotype 9) encoding Cas9 from S. mdqgym xgpsyk bkqxmm utgwzue pqsuhqk vwfzp hff dpmrmjw fuvc sxmglw mwiwi jtbqwme zvv ntuf fpeoegp